BAFF CHO
CBP74286
询 价
索取Protocol
产品描述
产品数据库
I. Background | |
BAFF(B 细胞激活因子,B Cell Activating Factor),也称为 TNFSF13B(Tumor Necrosis Factor Superfamily Member 13B)或 BLyS(B Lymphocyte Stimulator),是一种属 于肿瘤坏死因子(TNF)超家族的细胞因子。BAFF 主要由单核细胞、巨噬细胞、树突状 细胞和某些基质细胞产生,在 B 细胞的存活、增殖和成熟中起关键作用。 BAFF 通过与受体结合,调节 B 细胞的发育和功能,其过度表达与自身免疫性疾病 (如系统性红斑狼疮、类风湿性关节炎)密切相关。BAFF 有三个主要受体:BAFF-R (BR3)、TACI 和 BCMA。BAFF 与 BAFF-R 结合后,激活 NF-κB 和 PI3K/AKT 通路,促 进 B 细胞的存活和增殖;TACI 介导 BAFF 和 APRIL(另一种 TNF 家族成员)的信号传导, 调节 B 细胞的活化和抗体产生;BCMA 主要在浆细胞中表达,介导 BAFF 和 APRIL 的信 号传导,支持浆细胞的存活。BAFF 的过度表达与系统性红斑狼疮(SLE)、类风湿性关节 炎(RA)、干燥综合等自身免疫性疾病密切相关。 |
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II. Description | |
BAFF CHO 细胞,在 CHO-K1 细胞的基础上,稳定表达人 BAFF。 |
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III. Introduction | |
Expressed gene: | BAFF |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | F12K+10%FBS+600 μg/ml Hygromycin B |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Binding Assay,FACS |
IV. Representative Data![]() Figure 1. Recombinant BAFF CHO stably expressing BAFF. ![]() Figure 2. Blocking of BAFF induced TACI Effector Reporter Cell(C32) Activity by BAFF Neutralization Ab with BAFF CHO. |
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![]() Figure 2. Blocking of BAFF induced BAFFR Effector Reporter Cell(C18) Activity by BAFFR Blocking Ab with BAFF CHO. Blocking of BAFF induced BAFFR Effector Reporter Cell(C18) Activity by BAFF Blocking Ab with BAFF CHO. |