hMET/KLN205
CBPG0165
询 价
留 言
产品描述
产品数据库
I. Background | |
C-Met 属于受体酪氨酸激酶家族,是一种异二聚体,由高度糖基化的细胞外 50 kDa α 链和跨膜 140 kDa β链组成,通过二硫键连接在一起。作为一种天然的 HGF 受体,它表达 于各种上皮细胞的表面,并促进肿瘤的发生。与 HGF 结合后,两个亚基二聚化,导致两个 酪氨酸残基(Tyr1234 和 Tyr1235 )自磷酸化。酪氨酸残基的自磷酸化导致一个激活的多功 能停靠位点,该位点由 SH2 结构域、PTB 结构域和 Met 结合结构域 (MDB) 组成,该结 构域募集接头分子。 | |
II. Introduction | |
Host Cell: | KLN205 |
Expressed gene: | hMET |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | MEM+10%FBS+1%Non Essential Amino Acids(NEAA)+1mM Sodium Pyruvate(NaP)+10 μg/ml Blasticidin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
III. Description of Host Cell Line | |
Organism: | Mus musculus, mouse |
Tissue: | Lung |
Morphology: | Epithelial |
Growth Properties: | Adherent |
IV. Representative Data | |
Figure 1. Recombinant hMET/KLN205 stably expressing hMET. |
|