CD40/NFκB-Luc/HEK293
CBP74009
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| I. Background | |
| CD40 是肿瘤坏死因子受体超家族（TNFRSF）成员，作为免疫调节的关键分子，广泛 参与 B 细胞活化、抗原呈递细胞（APC）成熟及 T 细胞免疫应答。其配体 CD40L（CD154） 主要表达于活化的 T 细胞，通过 CD40-CD40L 互作桥接先天性与适应性免疫，是肿瘤免疫 治疗、自身免疫病及感染性疾病的热门靶点。 CD40 信号转导主要利用 TNF 受体相关因子的衔接蛋白，导致经典和非经典 NFκB 通 路、MAPK、PI3K 和 PLCγ的激活。它们的激活导致特征性下游效应，包括转录激活、细 胞骨架重排和细胞存活。 |
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| II. Description | |
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CD40 NFκB-Luc HEK293 报告基因药靶模型很好的模拟了体内 CD40 的信号转导过程， 原理见下图所示。
Figure 1. CD40 NFκB-Luc HEK293 细胞模型原理图
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CD40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-κB cell line, NFκB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10%FBS+1% NEAA+1 mM NaP+1 μg/ml Puromycin+100 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 2. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40. |
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